MicroVue Serum PYD EIA*

1.800.874.1517

English | Chinese

Intended Use	For Research Use Only. Not for use in diagnostic procedures.
Catalog Number	8019
Price	$940.00 (U.S. List Price)
Size	1 Kit (96 well)
Application	ELISA

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Description

For Research Use Only in the United States.
Not for use in diagnostic procedures.

MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.

In humans, the total pool of urinary Pyd is approximately 45% free while the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross-linked structures in excess of 10,000 Da. The proportion of free to total cross-links appears to be constant in healthy individuals and those with metabolic bone diseases or arthritis conditions, thus providing the rationale for measuring free Pyd.

References:

7 References

Krupski W, Tatara MR, Bury P, et al. Negative Effects of Total Gastrectomy on Bone Tissue Metabolism and Volumetric Bone Mineral Density (vBMD) of Lumbar Spine in 1-Year Study in Men. Cuce. G, ed. Medicine. 2016;95(7):e2817.

Xiong L., Xia W.-F., Tang F.-L., Pan J.-X., Mei L., Xiong W.-C. Retromer in Osteoblasts Interacts With Protein Phosphatase 1 Regulator Subunit 14C, Terminates Parathyroid Hormone’s Signaling, and Promotes Its Catabolic Response. EBioMedicine. 2016;9:45–60.

A. Lamora, M. Mullard, J. Amiaud et al., “Anticancer activity of halofuginone in a preclinical model of osteosarcoma: inhibition of tumor growth and lung metastases,” Oncotarget, vol. 6, no. 16, pp. 14413–14427, 2015.

Karolina Sieroń-Stołtny, Łukasz Teister, Grzegorz Cieślar, et al., “The Influence of Electromagnetic Radiation Generated by a Mobile Phone on the Skeletal System of Rats,” BioMed Research International, vol. 2015, Article ID 896019, 11 pages, 2015.

Decaris ML, Gatmaitan M, FlorCruz S, et al. Proteomic Analysis of Altered Extracellular Matrix Turnover in Bleomycin-induced Pulmonary Fibrosis. Molecular & Cellular Proteomics : MCP. 2014;13(7):1741-1752.

Xia, W.-F., et al., 2013. Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling. J. Cell Biol. 200 (6), 821–837

Huang Y, Zhao JX, Yan X, Zhu MJ, Long NM, et al. (2012) Maternal Obesity Enhances Collagen Accumulation and Cross-Linking in Skeletal Muscle of Ovine Offspring. PLOS ONE 7(2): e31691.

Features & Benefits

Feature	Benefit

Product Specifications

Product Spec Name	Product Spec Data
Format	ELISA
Test/kit	96 wells/plate
Sample type	Serum

Performance Characteristics

Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 µL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)

Species Reactivity

Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit

Assay Steps

Spin serum in Spinfilters (included) for 30 minutes
Add 50 µL Reagent 1 to plate
Add 25 µL serum, diluted Standards, diluted Controls
Add 75 µL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 µL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 µL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 µL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter