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Intended Use For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number 8050
Price $895.00 (U.S. List Price)
Size 1 Kit (96 well)
Application ELISA
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Description

For Research Use Only in the United States. Not for use in diagnostic procedures.

The MicroVue Klotho Assay is an enzyme immunoassay to quantitatively measure human α-soluble Klotho protein. See "Product Specifications" below for details specifications.

Klotho is a type-I transmembrane protein that is associated with many functions. A novel ß-glucuronidase, Klotho has been associated with phosphate regulation, organ protection, and aging, among others. Though a single gene, it has three major isoforms: the transmembrane form, a shed soluble form, and a truncated soluble form. The extracellular portion of the protein consists of two glycosyl hydrolase domains referred to as KL1 and KL2. Upon cleavage at the base of the extracellular domain the protein becomes the shed soluble form and is an active form that functions as a hormone. Importantly, Klotho is a receptor and a co-receptor of the phosphate regulation protein FGF-23. While Klotho/FGF-23 homeostasis has primarily been focused on phosphate and vitamin D regulation, continuing research is associating Klotho and FGF-23 with functionality that impacts the heart, bones, blood vessels, and parathyroid gland.

The MicroVue Klotho assay is a sandwich enzyme immunoassay in a microtiter plate format utilizing a capture monoclonal anti-Klotho antibody specific to the KL2 region coated on the plate and a detection mouse monoclonal anti-Klotho antibody specific to the KL1 region.

Features & Benefits

FeatureBenefit

Monoclonal development of antibodies specific to KL1 and KL2 domains.

Direct detection of α-soluble Klotho protein.

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Assay Time

3 hours 45 minutes

Tests/kit

96 wells/plate

Sample Type

Plasma and Serum

Sample Volume

100 µL

LOD

0.04 ng/mL

LLOQ

0.13 ng/mL

ULOQ

18.00 ng/mL

Ordering Information

Catalog NumberDescription
8050

ELISA Kit, controls included

Product Documentation

Product documentation is best viewed in Internet Explorer
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