For Research Use Only. Not for use in diagnostic procedures.
The MicroVue Creatinine assay is offered as a convenience for researchers with no other means to perform the test. Urine creatinine is required to normalize the MicroVue urine resorption assays, DPD, PYD, HP, urine Total Dpd.
Creatinine is a molecule with a molecular weight of 113.1 g/mol. Creatinine is found in muscle tissues and is excreted into the circulation at a constant rate. It is removed from the plasma by globular filtration and excreted into the urine. The MicroVue Creatinine assay is a quantitative, colorimetric assay based on a modified Jaffe method where alkaline picrate forms a colored solution in the presence of creatinine.
Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
Format | Colorimetric assay based on Jaffe method |
Test/kit | 96 wells/plate |
Sample type | Urine |
About the Assay
Format
96-well microplate with reagents sufficient to test 42 samples in duplicate
Species Reactivity
Creatinine values from non-human species have not been evaluated with the MicroVue Creatinine kit.
Assay Steps
Add 50 µL 1:40 diluted Standards, Controls and samples
Add 150 µL Working Color Solution
Incubate 30 minutes at 18°C to 28°C
Read Optical Density at 490 nm
Ordering Information
| Catalog Number | Description | Kit Size / Case Size |
|---|---|---|
8009 | ELISA Kit | 96 Wells/Plate |
