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The Lyra RSV + hMPV Assay is a multiplex real-time PCR assay for the qualitative detection and identification of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) viral RNA extracted from nasal swabs and nasopharyngeal swab specimens. This real time RSV + hMPV test detects RSV and hMPV and is able to differentiate between them.

A multiplex real-time RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for RSV, hMPV and the Process Control (PRC). Identification of RSV and hMPV and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the NS2 and polymerase genes of RSV and polymerase gene of hMPV and the PRC.

The Lyra RSV + hMPV Assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra RSV + hMPV RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.

The Lyra RSV + hMPV Assay is one in a growing menu of tests and intended to aid in the differential diagnosis of RSV and hMPV infections in humans.

Features & Benefits


One-step reagent set-up

Rehydration solution is simply added to the lyophilized Master Mix


Results in less than 70 minutes after extraction

Easy training

Simplified and uniform workflow with standard pipetting volumes

Refrigerated storage

No freezer needed. 2°C to 8°C storage

Long shelf life

2 years from date of manufacture

Room temperature set up

No ice or cooling block required

Flexible 96-test format

Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls

Product Specifications

Product Spec NameProduct Spec Data
Sample type

Throat samples from symptomatic patients

Time to results

Results in less than 70 minutes

Reagent storage conditions

2°C to 8°C

Controls storage conditions

2°C to 8°C


Strep Groups C and G: 95.7%


Strep Groups C and G: 98.3%

Positive Percent Agreement*

hMPV: 98.0%

Negative Percent Agreement*

hMPV: 98.9%

Sample volume needed for extraction

180 µL

Shelf life

24 months

*Data for Applied Biosystems™ 7500 Fast Dx. Refer to Package Insert for additional performance claims.

Ordering Information

Catalog NumberDescriptionKit Size / Case Size

Assay Kit

96 tests

Quidel Molecular RSV + hMPV Control Set

10 reactions

To better serve your molecular diagnostic needs Quidel offers two instruments