The Lyra Direct HSV 1+2/VZV Assay is a multiplex in vitro test that detects and differentiates Herpes simplex virus type 1, Herpes simplex virus type 2, and Varicella-zoster virus nucleic acids isolated and purified from cutaneous or mucocutaneous swab specimens obtained from symptomatic patients.
A multiplex real-time PCR reaction is carried out under optimized conditions in a single tube generating amplicons for HSV-1, HSV-2, VZV, and the Process Control (PRC). Identification of HSV-1, HSV-2, VZV, and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the genomes of HSV-1, HSV-2, VZV and the PRC.
The Lyra Direct HSV 1+2/VZV Assay has one-step reagent set-up and a three-step sample prep. Test results are available in less than 70 minutes. The Lyra Direct HSV 1+2/VZV Assay is available for use on Applied Biosystems® 7500 Fast Dx, QuantStudio™ Dx Real-Time PCR Instrument, and Cepheid® SmartCycler® II.
The Lyra Direct HSV 1+2/VZV Assay is one in a growing menu of PCR tests and is used as an aid in the differential diagnosis of Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) and Varicella-zoster virus (VZV) also known as Human herpes virus 3. VZV is the causative agent for chicken pox and shingles.
Features & Benefits
| Feature | Benefit |
|---|---|
One-step reagent set-up | Rehydration solution is simply added to the lyophilized Master Mix |
Speed | Results in less than 60 minutes |
Easy training | Simplified and uniform workflow with standard pipetting volumes |
Refrigerated storage | No freezer needed. 2°C to 8°C storage |
Long shelf life | 2 years from date of manufacture |
Room temperature set up | No ice or cooling block required |
Flexible 96-test format | Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls. Sample preparation kit includes all consumables |
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
Sample type | Cutaneous or mucocutaneous swab specimens |
Time to results | Less than 60 minutes |
Reagent storage conditions | 2°C to 8°C |
Controls storage conditions | 2°C to 8°C |
Sample volume needed for extraction | 100 µL |
Purified nucleic acid volume needed to testing | 5 µL |
Sensitivity* | Cutaneous: HSV-1 100.0%, HSV-2 97.1%, VZV 96.3%; Mucocutaneous: HSV-1 95.1%, HSV-2 97.9%, VZV 100.0% |
Specificity* | Cutaneous: HSV-1 98.8%, HSV-2 96.7%, VZV 96.4%; Mucocutaneous: HSV-1 98.2%, HSV-2 97.1%, VZV 99.3% |
Shelf life | 24 months |
*Data for Applied Biosystems™ 7500 Fast Dx. Refer to Package Insert for additional performance claims.
Ordering Information
| Catalog Number | Description | Kit Size / Case Size |
|---|---|---|
M102 | Assay Kit | 96 tests |
M121 | Quidel Molecular HSV 1+2/VZV Control Set | 10 reactions |
Product Documentation
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