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The Lyra Parainfluenza Virus Assay is a multiplex real-time PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs from symptomatic patients. The assay has been shown to detect and differentiate parainfluenza virus types 1, 2 and 3.

During the test, a multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of PIV-1, PIV-2, PIV-3 and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the genomes of PIV-1, PIV-2, PIV-3 and the PRC.

The Lyra Parainfluenza Virus Assay has a convenient, one-step reagent set up performed at room temperature on the bench top, without the need for ice or other cooling accessories.  Moreover, optimized cycling conditions make results available in less than 75 minutes following extraction to aid in timely diagnosis and treatment. Lyra Parainfluenza Virus RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument.

The Lyra Parainfluenza Virus Assay provides  the flexibility to  customize respiratory panels with other Lyra Real-Time PCR respiratory assays (Lyra Influenza A+B, Lyra hMPV+RSV, Lyra Adenovirus) that are run under the same cycling conditions in order to adapt to the  shifting demands caused by  variable local or seasonal disease prevalence.

Features & Benefits


One-step reagent set-up

Rehydration solution is simply added to the lyophilized Master Mix


Results in less than 75 minutes after extraction

Easy training

Simplified and uniform workflow with standard pipetting volumes

Refrigerated storage

No freezer needed. 2°C to 8°C storage

Long shelf life

2 years from date of manufacture

Room temperature set up

No ice or cooling block required

Flexible 96-test format

Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls

Multi-well multiplex panels

Standardized thermocycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results

Product Specifications

Product Spec NameProduct Spec Data
Sample type

Nasal swab, Nasopharyngeal swab

Time to results

Less than 75 minutes after extraction

Reagent storage conditions

2°C to 8°C

Controls storage conditions

2°C to 8°C


PIV-1: 100%; PIV-2:100%; PIV-3:100%


PIV-1: 99.8%; PIV-2:100%; PIV-3:99.6%

Positive Percent Agreement*

PIV-1: 100%; PIV-2:100%; PIV-3:100%

Negative Percent Agreement*

PIV-1: 98.8%; PIV-2:94.0%; PIV-3:100%


PIV-1: 2.50 x 100; PIV-2: 2.50 x 102; PIV-3:8.00 x 101 (TCID50/mL)

Sample volume needed for extraction

180 µL

Purified nucleic acid volume needed to testing

5 µL

Shelf life

24 months

*Refer to Package Insert for additional performance claims.

Ordering Information

Catalog NumberDescriptionKit Size / Case Size

Assay Kit

96 tests

Quidel Molecular Parainfluenza Virus Control Set

10 reactions

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