The Lyra Adenovirus Assay is a real-time PCR in vitro diagnostic assay for the qualitative detection of human Adenovirus (HAdV) viral DNA which has been isolated from nasal and nasopharyngeal swabs obtained from individuals exhibiting signs and symptoms of acute respiratory infections. The test detects, but does not differentiate, HAdV species (A, B, C, D, E, and F) or serotypes.
During the test, a multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of adenovirus (HAdV) and the PRC occur by the use of target-specific primers and fluorescent-labeled probes which hybridize conserved regions in the genomes of adenovirus and the PRC.
The Lyra Adenovirus Assay has a convenient, one-step reagent set up performed at room temperature on the bench top. Moreover, optimized cycling conditions make results available in less than 75 minutes following extraction to aid in timely diagnosis and treatment. Lyra Adenovirus RT-PCR is available for use on the Applied Biosystems®7500 Fast Dx Real-Time PCR Instrument.
The Lyra Adenovirus Assay, when used with other Lyra assays, provides the flexibility to customize respiratory panels with other Lyra Real-Time PCR Respiratory assays in order to adapt to the shifting demands caused by variable local or seasonal disease prevalence.
Features & Benefits
| Feature | Benefit |
|---|---|
One-step reagent set-up | Rehydration solution is simply added to the lyophilized Master Mix |
Speed | Results in less than 75 minutes after extraction |
Easy training | Simplified and uniform workflow with standard pipetting volumes |
Refrigerated storage | No freezer needed. 2°C to 8°C storage |
Long shelf life | 2 years from date of manufacture |
Room temperature set up | No ice or cooling block required |
Flexible 96-test format | Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls |
Multi-well multiplex panels | Standardized thermocycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results |
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
Sample type | Nasal swab, Nasopharyngeal swab |
Time to results | Less than 75 minutes after extraction |
Reagent storage conditions | 2°C to 8°C |
Controls storage conditions | 2°C to 8°C |
Sensitivity* | 100% |
Specificity* | 95.5% |
Positive Percent Agreement* | 100% |
Negative Percent Agreement* | 98.7% |
LOD | A/31: 8.00 x 10-2; B/3: 8.00 x 10-2; C/1: 8.00 x 10-2; D/19: 1.61 x 101; E/4: 1.00 x 100; F/41: 3.20 x 10-2 (TCID50/mL) |
Sample volume needed for extraction | 180 µL |
Purified nucleic acid volume needed to testing | 5 µL |
Shelf life | 24 months |
*Refer to Package Insert for additional performance claims.
Ordering Information
| Catalog Number | Description | Kit Size / Case Size |
|---|---|---|
M113 | Assay Kit | 96 tests |
M110 | Quidel Molecular Adenovirus Control Set | 10 reactions |
Product Documentation
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