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Helicase Dependent Amplification Assays

Helicase Dependent Amplification (HDA) is an isothermal DNA amplification method that is similar to conventional PCR, but utilizes the action of a thermostable helicase enzyme, rather than heat to separate the nucleic acids and enable labeled primers to anneal to the DNA template and elongate under the action of the polymerase.
This allows DNA replication to occur at a single uniform temperature (64°C) with a simple heat block, rather than a series of varying time and temperature conditions utilized by expensive real-time thermocyclers.
The process supplies accurate molecular test results that you can see, achieved without the need for specialized equipment or complex software for data interpretation.


Step 1
Specific primers bind to target sequences that have been separated by the helicase.

Step 2
Specific DNA probes labeled with a quencher on one end and a fluorophore on the other end bind to the single-stranded biotinylated amplicons.

Step 3
Upon annealing to the amplicons, the fluorescence probes are cleaved and the fluorescence signal increases due to physical separation of fluorophore from quencher.


Quidel has developed the Solana assays that use Helicase Dependent Amplification to provide access to all the benefits of molecular testing for any size laboratory.


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