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Helicase Dependent Amplification Assays

Helicase Dependent Amplification (HDA) is an isothermal DNA amplification method that is similar to conventional PCR, but utilizes the action of a thermostable helicase enzyme, rather than heat to separate the nucleic acids and enable labeled primers to anneal to the DNA template and elongate under the action of the polymerase.
 
This allows DNA replication to occur at a single uniform temperature (64°C) with a simple heat block, rather than a series of varying time and temperature conditions utilized by expensive real-time thermocyclers.
 
The process supplies accurate molecular test results that you can see, achieved without the need for specialized equipment or complex software for data interpretation.
 

Solana

Step 1
Specific primers bind to target sequences that have been separated by the helicase.

Step 2
Specific DNA probes labeled with a quencher on one end and a fluorophore on the other end bind to the single-stranded biotinylated amplicons.

Step 3
Upon annealing to the amplicons, the fluorescence probes are cleaved and the fluorescence signal increases due to physical separation of fluorophore from quencher.

 

Quidel has developed the Solana assays that use Helicase Dependent Amplification to provide access to all the benefits of molecular testing for any size laboratory.

 

  • Solana Bordetella Complete Assay

    The Solana Bordetella Complete Assay is an in vitro diagnostic test for the qualitative detection of Bordetella pertussis and Bordetella...

  • Solana C. difficile Assay

    The Solana C. difficile Assay is an in vitro diagnostic test for the direct, qualitative detection of the Clostridioides (Clostridium) difficile...

  • Solana GAS Assay

    The Solana GAS Assay is a rapid in vitro diagnostic test for the qualitative detection of Group A β-hemolytic Streptococcus (Streptococcus...

  • Solana GBS Assay

    The Solana GBS Assay is an in vitro diagnostic test for the direct, qualitative detection of Group B Streptococcus from vaginal/rectal swabs...

  • Solana Respiratory Viral Panel

    Solana Respiratory Viral Panel (RVP) is a rapid qualitative in vitro diagnostic test for the detection and differentiation of respiratory syncytial...

  • Solana SARS-CoV-2 Assay

    The Solana SARS-CoV-2 Assay is an isothermal Reverse Transcriptase - Helicase-Dependent Amplification (RT-HDA) assay intended for the qualitative detection...

  • Solana Strep Complete Assay

    The Solana Strep Complete Assay is a rapid in vitro diagnostic test for the qualitative detection of Group A β-hemolytic Streptococcus (...

  • Solana Trichomonas Assay

    The Solana Trichomonas Assay is a rapid in vitro diagnostic test for the qualitative detection of nucleic acids isolated from clinician-collected vaginal...

Herpes Family Testing

  • Solana HSV 1+2/VZV Assay

    The Solana HSV 1+2/VZV Assay is a multiplex in vitro diagnostic test for the detection and differentiation of Herpes simplex virus 1 (HSV-1),...

Influenza

  • Solana Influenza A+B Assay

    The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and...

Respiratory Family Testing

  • Solana RSV + hMPV Assay

    The Solana RSV + hMPV Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of Respiratory Syncytial Virus (...

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