Fluorescent Immunoassays (FIA)
Fluorescent Immunoassays are simply a different type of immunoassay. The key variable is the biochemical technique used for detecting the binding of the “detection” antibody and the analyte molecule. The advantages of a Fluorescent detection system have been known for many years. These include higher sensitivity detection of the analyte, simplified reagents and simpler assay designs. Several breakthroughs have occurred over the past few years that have enabled the implementation of a fluorescent based immunoassay system at the point of care.
A modern fluorescent based immunoassay uses as the detection reagent a fluorescent compound which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength. The difference between the wavelength of the excitation light and the emission light is called the Stokes shift. The greater the shift or difference in the wavelength the less interference there will be by having the excitation light detected as part of the emission light. Recently a number of technical improvements have occurred that has enabled the implementation of a high sensitivity immunoassay system. These include the availability of narrow wavelength low cost light sources, newer more stable fluorophores that have very wide Stokes shifts, stable solid state light detectors and microprocessors to process and analyze the data from each test.
When a fluorescent detection system is linked to a lateral flow assay and matched with a powerful yet low cost analyzer like the Quidel Sofia or Sofia 2, the result is improved assay performance, the opportunity for walk away testing along with the elimination of misinterpretation often associated with visually-read point-of-care assays.
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The Quidel Triage BNP Test is a rapid, point-of-care fluorescence immunoassay used with the Quidel Triage MeterPro. The test is used to measure B-type...
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The Quidel Triage BNP Test for the Beckman Coulter® Access Family of Immunoassay Systems empowers higher throughput BNP with rapid testing and...
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The Quidel Triage Cardiac Panel is a rapid, point of care fluorescence immunoassay used with the Quidel Triage MeterPro. This test is designed to determine...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Quidel Triage Cardio2 Panel is a fluorescence immunoassay...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Quidel Triage Cardio3 Panel is a flourescence...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Quidel Triage Profiler SOB Panel is a fluorescence...
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The Quidel Triage TOX Drug Screen is a fluorescence immunoassay to be used with the Quidel Triage MeterPro for the qualitative determination of the presence...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Quidel Triage Troponin I Test is a next generation...
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Sofia 2 is a small bench top analyzer that uses advanced fluorescence detection with an ultraviolet LED energy source.
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Sofia is a small bench top analyzer that uses advanced fluorescence detection with an ultraviolet LED energy source.
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Sofia hCG Fluorescent Immunoassay (FIA), with Kinetic Check technology uses advanced immunofluorescence-based lateral-flow technology to detect human...
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For use with Sofia 2 and Sofia. The Sofia Influenza A+B Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Sofia Legionella Fluorescent Immunoassay (FIA) uses...
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For use with Sofia 2 and Sofia. The Sofia Lyme Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow technology...
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For use with Sofia 2 and Sofia. The Sofia RSV Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow technology...
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NOT AVAILABLE FOR SALE IN THE U.S.
The Sofia S. pneumoniae Fluorescent Immunoassay (FIA)...
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The Sofia Strep A Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow technology to detect Group A Streptococcal antigens from...
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For use with Sofia 2 and Sofia. The Sofia Strep A+ Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow...
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Tools to support the daily capture of surveillance data for Influenza and RSV.