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Human Sclerostin HS ELISA

For Research Use Only. Not for use in diagnostic procedures.

Sclerostin is the protein product of the SOST gene, which is located at 17q12-21 and highly conserved across vertebrate species. The highest expression of sclerostin throughout the adult skeleton has been observed in hypertrophic chondrocytes and osteocytes. Sclerostin blocks canonical Wnt signaling by binding to the Wnt coreceptors LRP5/6, inhibiting bone formation by regulating osteoblast function and promoting osteoblast apoptosis. Sclerostin also antagonizes bone morphogenetic protein (BMP) action (e.g. osteoblast differentiation), but does not inhibit direct BMP-induced responses. Sclerostin expression is down-regulated by Parathyroid hormone (PTH), as well as, by the mechanical stimulation of bone. Reduced expression of sclerostin can result in van Buchem disease, while a complete absence results in Sclerosteosis. Patients affected by Sclerosteosis show progressive hyperostosis and sclerosis of the skull, mandible and all long bones. Bone mineral density (BMD), bone volume, bone formation rate, and bone strength are significantly increased, while overall skeletal morphology appears to be normal. A predominance of sclerostin causes reduced bone quality (Osteoporosis pseudoglioma (OPPG) syndrome). Down-regulation of sclerostin might be used as a treatment for diseases such as osteoporosis, promote osseointegration of implants, prevent periprosthetic bone loss, or treat non-union in fractures. Local enhancement of sclerostin expression might be used to prevent cancer metastasis and minimize further expansion of ectopic bone formation.

  • Sclerosteosis
  • Van Buchem disease
  • Osteoporosis pseudoglioma (OPPG) syndrome

An enzyme immunoassay for the quantitation of sclerostin in human serum.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Serum

Performance Characteristics

Method: Sandwich
Analyte: Sclerostin
Specimen: Serum
Specimen Volume: 25 µL
Sensitivity: 0.008 ng/mL
Assay Range: 0-3 ng/mL
Precision CVs within-run: 3.7%-4.2%
Precision CVs between-run: 4.3%-4.8%
Incubation Time: 4.5 hours

Ordering Information

Catalog NumberDescription
TE1023HS

96 Wells/Plate

Product Documentation