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Human Leptin ELISA

For Research Use Only. Not for use in diagnostic procedures.

The Human Intact Proinsulin kit is an enzyme immunoassay for the quantitation of leptin in human serum, plasma and other experimental samples.

Leptin, the product of the ob gene, is a recently discovered proteohormone. It is almost exclusively produced by differentiated adipocytes and is thought to play a key role in the regulation of body weight. Leptin has an influence on the central nervous system, mainly on the hypothalamus, by suppressing food ingestion and increasing energy consumption. Beside its influence on food intake, leptin has been shown to have a strong effect on reproduction and a number of metabolic and endocrine axes. As leptin is of great importance for reproductive functions, infertility may be due to inadequate leptin production. The most important variable determining the circulating leptin concentration is the body fat mass as leptin level and fat mass increase exponentially. Due to its pleiotropic effects, leptin is a valuable parameter with regard to:

  • Metabolic syndrome
  • Adiposity
  • Cachexia and other metabolic disorders
  • Eating disorders

An enzyme immunoassay for the quantitation of leptin in human serum, plasma, and other experimental samples.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Various

Performance Characteristics

Method: Sandwich
Analyte: Leptin
Specimen: Serum, heparin plasma, urine, CSF, cell culture
Specimen Volume: 20 µL
Sensitivity: 0.2 ng/mL
Assay Range: 1-100 ng/mL
Precision CVs within-run: 5.4%-6.8%
Precision CVs between-run: 6.8%-8.3%
Incubation Time: 2 hours

Ordering Information

Catalog NumberDescription
TE1016

96 Wells/Plate

Product Documentation