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For Research Use Only. Not for Use in Diagnostic Procedures

The MicroVue YKL-40 is a test to measure YKL-40, also known as human cartilage 39 (HC gp-39), in serum and human cell cultures. Though the role of YKL-40 is unknown at present, its pattern of expression and observed associations with various disease activities suggest it plays a role in tissue remodeling.

YKL-40 — also known as human cartilage glycoprotein 39 (HC gp-39) is a 40 KD glycoprotein first described in whey secretions of nonlactating cows. Subsequently, its production by chondrocytes, synovial cells, activated macrophages, neutrophils, and osteosarcoma cells (MG-63) has been reported. It has both heparin and chitin binding domains and appears to be an autoantigen in rheumatoid arthritis.

Early studies suggest YKL-40 is useful for researching:

  • Rheumatoid Arthritis
  • Early polyarthritis
  • Osteoarthritis
  • Giant cell arteritis
  • Liver fibrosis
  • Breast cancer
  • Colorectal cancer

Recently, serum YKL-40 has been investigated as a new marker for cancer. Elevated serum YKL-40 is associated in many cancers studied to date, including cancer of the colon, lung, prostate, ovary, and breast.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Tests/kit

96-well/plate

Sample type

Serum, plasma

Performance Characteristics

Method: Sandwich
Analyte: YKL-40
Specimen: Serum, Plasma
Specimen Volume: 20 µL
LOD*: 5.4 ng/mL
LLOQ*: 15.6 ng/mL
HLOQ*: 300 ng/mL
Precision CVs within-run: 5.6%-6.6%
Precision CVs between-run: 6%-7%
Incubation time: 3 hours
Cross-reactivity: YKL-40/HC gp-39 100%

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate

Species Reactivity

Baboon, Rhesus and Cynomolgus Macaque, and Human

Assay Steps

Add 20 µL Standards, Controls, and samples
Add 100 µL Capture Solution
Incubate 60 mintues at room temperature
Wash 4 times with 1X Wash Buffer
Add 100 µL Enzyme Conjugate
Incubate 60 minutes at room temperature
Wash 4 times with 1X Wash Buffer
Add 100 µL Working Substrate Solution
Incubate 60 minutes at room temperature
Add 100 µL Stop Solution
Read Optical Density at 405 nm
Data Reduction: Linear Curve Fit

Ordering Information

Catalog NumberDescription
8020

ELISA Kit