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Intended Use For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number 8033 (US), 8036 (XUS)
Price $870.00 (U.S. List Price)
Size 1 Kit (96 well)
Application ELISA
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Description

For Research Use Only in the United States. Not for Use in Diagnostic Procedures

The MicroVue TRAP5b Assay is an immunocapture enzyme assay for the determination of tartrate-resistant acid phosphatase isoform 5b (TRAcP 5b) in human serum and plasma.

TRAP5b (serum band 5 tartrate-resistant acid phosphatase, TRAcP 5b; EC 3.1.3.2) is a 35-37 kDa glycoprotein. TRAP5b is typically expressed in proportion to osteoclast activity and is secreted into circulation. As a result, serum TRAP5b is considered a potentially useful marker for bone resorption. The MicroVue TRAP5b EIA detects the enzyme activity of bound TRAP5b in experimental samples.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit:

96 wells/40 (in duplicate)

Sample type:

Plasma and serum

About the Assay

Format

96 well assay plate

Species Reactivity

Reactive with human derived TRAP5b only.
No reactivity detected with Cow, Chicken, Goat, Mouse, Rabbit, Rat, Sheep, Guinea Pig, Baboon, Cynomolgus Monkey, Rhesus Macaque

Assay Steps

Add 100 µL of Sample Diluent
Add 50 µL of Calibrator, Controls and Specimens
Seal the microplate
Incubate for 60 minutes at room temperature on a microplate shaker
Wash the microplate wells three times with Wash Buffer
Add 100 µL of Substrate Solution
Seal the microplate
Incubate for 60 minutes at 37°C
Add 50 µL of Stop Solution
Read OD at 405 nm
Data Reduction Quadratic

Ordering Information

Catalog NumberDescription
8033 US

ELISA Kit

8036 XUS

ELISA Kit