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For Research Use Only. Not for use in diagnostic procedures.

The MicroVue Helical Peptide is a test to measure type I collagen helical peptide α1(I) 620-633 in urine, a marker for researching collagen degradation.

Type I collagen is degraded during bone resorption, releasing into circulation peptides of various molecular weights that are further degraded and/or released into urine. A peptide consisting of residues 620-633 derived from the helical region of the α1 chain was isolated from the urine of a patient with Paget's disease.

 

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Urine

Performance Characteristics

Method: Competitive
Analyte: Helical Peptide α1(I) 620-633
Specimen: Urine
Specimen Volume: 20 µL
LOD*: 8 µg/L
LLOQ*: 20.2 µg/L
HLOQ*: 2514 µg/L
Precision CVs within-run: 4%-8%
Precision CVs between-run: 7%-17%
Incubation Time: 19-25 Hours
Cross-reactivity: Helical peptide α1(I) 620-633 100% Intact collagen I, II, III, IV < 1% Homologous peptides (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Helical peptide α1(I) 620-633; no reactivity with intact collagen types I, II, III, IV or mixtures of amino acids; 90% reactivity with α1(III) homologous peptide; no reactivity with α1(I) or α2(II) homologous peptides

Species Reactivity

Rat, Mouse, Rabbit, Guinea Pig, Cat, Dog, Pig, Goat, Sheep, Cow, Horse, Rhesus Macaque, Cynomolgus Monkey, Baboon, Chimpanzee, Human Cell Culture

Assay Steps

Add 20 µL Standards, Controls, and samples
Add 150 µL Enzyme Conjugate

  • Incubate 18-24 hours at 2°C to 8°C
  • Wash 3 times with 1x Wash Buffer
  • Add 150 µL Substrate
  • Incubate 1 hour at room temperature
  • Add 100 µL Stop Solution
  • Read absorbance at 405 nm
  • Data Reduction: 4 Parameter
  • Correct for creatinine concentration

Ordering Information

Catalog NumberDescriptionKit Size / Case Size
8022

ELISA Kit

96 Wells/Plate