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Intended Use For Research Use Only. Not for use in diagnostic procedures.
Catalog Number A039 (US), A040 (XUS)
Price $636.00 (U.S. List Price)
Size 1 Kit (96 well)
Application ELISA
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Description

For Research Use Only. Not for use in diagnostic procedures.

The alternative complement pathway provides innate protection against microbial agents in the absence of specific antibody.1-5 The activation of this complement pathway can be triggered by a variety of substances including microbial polysaccharides or lipids, gram negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the alternative complement pathway may contribute directly to tissue damage.

Factor H is involved in the regulation of the alternative pathway of complement. In blood, activation of C3, under normal conditions, is kept at a low level by the control proteins, Factor H and Factor I. Factor H functions in two ways to inactivate the C3bBb enzyme: (1) it accelerates the dissociation of Bb from C3b; and (2) it serves as a cofactor for Factor I, a serine protease, which cleaves C3b into iC3b, which can no longer form the C3 convertase with Factor B.6

Factor H also regulates the spontaneous fluid-phase activation of the alternative complement pathway by C3b-like forms of C3 that continuously arise in plasma and serum. Therefore, when concentrations of Factor H fall below normal levels, there is rapid fluid-phase activation and consumption of complement components both in vivo and in vitro.7

Known levels of Factor H aid in the diagnosis of several disease states such as atypical Hemolytic-Uremic Syndrome (aHUS), age-related macular degeneration, and dense deposit disease. Complement Factor H has been implicated in the research of many autoimmune diseases. Studies have included using Factor H as a serum biomarker of multiple sclerosis disease state, as a therapy for renal diseases associated with Factor H abnormalities, and as a camouflage to tumor cells for protection against the host immune system. This broad range of testing gives Factor H an appeal to many types of research.9

References

  1. Pangburn, M.K. and Müller-Eberhard, H.J. The alternative pathway of complement. Springer Semin. Immunopathol. 1984;7:163.

  2. Ratnoff, W.E., Fearon, D.T., and Austen, K.F. The role of antibody in the activation of the alternative complement pathway. Springer Semin. Immunopathol. 1983;6:361.

  3. Male, D., In Focus; Complement 2nd edition. 1995; 16-18

  4. Ferriera, V., Pangburn, M., Cortes, C., Complement Control Protein Factor H: The Good, The Bad, and the Inadequate. Mol Immunol. 2010 August;47(13): 2187-2197

  5. Kishore, U., Sim, R., Factor H as a Regulator of the Classical Pathway Activation. Immunobioloby 2012 Volume 217, Issue 2; 162-168

  6. Kopp, A., Hebecker, M., Svobodova, E., Jozsi, M. Factor H: A Complement Regulator in health and Disease, and a Mediator of Cellular Interactions. Biomolecules. 2012; 2 46-75

  7. Schreiber, R.D. and Müller-Eberhard, H.J. New developments in the activation of the alternative pathway of complement. In: Immunoassays: Clinical Laboratory Techniques for the 1980’S. Alan R. Liss, Inc., New York. 1980: p.411.

  8. Gotze, O. and Müller-Eberhard, H.J. The alternative pathway of complement activation. Adv. Immunol. 1976;24:1.

  9. Fearon, D.T. and Austen, K.F. Current concepts in immunology: the alternative pathway of complement – a system for host resistance to microbial infection. New Engl. J. Med. 1980;303: 259

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Assay time

150 minutes

Test/kit

96 wells/plate

Sample Type

Various

Ordering Information

Catalog NumberDescription
A039

96 Wells/Plate, control included

A040

96 Wells/Plate, control included