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For Research Use Only in the United States. Not for use in diagnostic procedures.

The MicroVue CICP assay measures type I C-terminal collagen propeptide (CICP) in serum. CICP may be useful as a biochemical indicator of collagen production and growth.

Collagen, the triple-helical molecule which forms the fibrous framework of all connective tissues, is synthesized as procollagen, a larger precursor molecule. Procollagen consists of mature collagen with extension peptides at both the amino and carboxy termini. These extension peptides, or propeptides, are cleaved from the collagen molecule by specific proteases prior to incorporation of collagen into a growing collagen fibril. The release of these peptides into the circulation provides a stoichiometric representation of the production of collagen.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Serum

Performance Characteristics

Method: Sandwich
Analyte: Collagen type 1 C-terminal propeptide
Specimen: Serum
Specimen Volume: 25 µL
LOD*: 0.2 ng/mL
LLOQ*: 1 ng/mL
HLOQ*: 80 ng/mL
Precision CVs within-run: 5%-7%
Precision CVs between-run: 5%-7%
Incubation Time: 4 Hours
Cross-reactivity: CICP 100%

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate.

Species Reactivity

Rabbit, Cynomolgus Monkey, Sheep, Human Cell Culture
No Recognition of Rat, Dog, Goat, or Horse

Assay Steps

Add 100 µL Standards, Controls and 1:12 diluted samples
Incubate 120 ± 5 minutes at 18°C to 25°C
Wash 3 times with 1X Wash Buffer
Add 100 µL Rabbit anti-CICP
Incubate 45-50 minutes at 18°C to 25°C
Wash 3 times with 1X Wash Buffer
Add 100 µL Enzyme Conjugate
Incubate 45-50 minutes at 18°C to 25°C
Wash 3 times with 1X Wash Buffer
Add 100 µL Working Substrate Solution
Incubate 30-35 minutes at 18°C to 25°C
Add 50 µL Stop Solution and read Optical Density at 405 nm
Data Reduction: 4 Parameter

Ordering Information

Catalog NumberDescription
8003

ELISA Kit