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14-3-3 eta ELISA Kit*

For Research Use Only. Not for use in diagnostic procedures.

The JOINTstat® 14-3-3η (14-3-3eta) ELISA is intended for research use only for the quantitative measurement of 14-3-3η in serum and plasma samples.

In the non-diseased state, 14-3-3η is an intracellular protein and behaves as a molecular chaperone regulating the activity and sub-cellular distribution of intracellular proteins such as kinases. Through these intermolecular interactions, intracellular 14-3-3η regulates a variety of biological processes including cell-cycle regulation, transcription, cytoskeletal structure and protein trafficking.1, 2

In the extracellular environment, 14-3-3η engages a cell-surface receptor and has been demonstrated to lead to the activation of specific signaling pathways. 14-3-3η-mediated activation of these pathways results in the induction of cytokines central to the rheumatoid arthritis (RA) inflammatory process as well as the up-regulation of matrix metalloproteinases (MMPs), enzymes that are key mediators of the joint damage process.3 In RA, 14-3-3η is externalized into the synovial fluid and subsequently enters the general circulation where it can be measured in serum and plasma.4,5 Serum 14-3-3η levels correlate strongly with levels of MMP-1 and MMP-3, and higher levels of 14-3-3η are observed in the serum of RA patients with erosive disease as compared to non-erosive disease.4 Taken together, these mechanistic and clinical data implicate 14-3-3η in the pathogenesis of RA.

References

1. H. Aitken, H. Baxter, T. Dubois S. Clokie, S. Mackie, K. Mitchell, A. Pended, and E. Zemilchkova. 2002. 14-3-3 Proteins in Cell Regulation. Biochem. Soc. Trans. 30(pt4): 351-360.

2. Fu, R.R. Subramanian, and S.C. Masters. 2000. 14-3-3 Proteins: Structure, Function, and Regulation. Annu. Rev. Pharmacol. Toxicol. 40: 617–47.

3. W. P. Maksymowych, D. van der Heijde, C. F. Allaart, R. Landewé, G. Boire, P. P. Tak, Y. Gui, A. Ghahary, R. Kilani, A. Marotta. 2014. 14-3-3η is a novel mediator associated with the pathogenesis of rheumatoid arthritis and joint damage. Arthritis Res. Ther. 16(2): R99.

4. R.T. Kilani, W.P. Maksymowych, A. Aitken, G. Boire, Y. St-Pierre, Y. Li, and A. Ghahary. 2007. Detection of High Levels of 2 Specific Isoforms of 14-3-3 Proteins in Synovial Fluid from Patients with Joint Inflammation. J. Rheum. 34: 1650-57.

5. W.P. Maksymowych, S.J. Naides, V.Bykerk, K.A. Siminovitch, D. van Schaardenburg, M. Boers, R. Landewé, D. van der Heijde, P.P Tak, M.C. Genovese, M.E. Weinblatt, E. Keystone, O.S. Zhukov, R.W Abolhosn, J.M. Popov, K. Britsemmer, A. van Kuijk, and A. Marotta. 2014. Serum 14-3-3η is a Novel Marker that Complements Current Serological Measurements to Enhance Detection of Patients with Rheumatoid Arthritis. J. Rheum. 41(11): 2104-13.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Assay Time

4 hours

Test/kit

96 wells/plate

Sample type

Serum/Plasma

Ordering Information

Catalog NumberDescription
1358000

ELISA Kit

1358200

14-3-3 eta Negative Serum Control (liquid, 25 µL)

1358300

14-3-3 eta Low Positive Serum Control (liquid, 25 µL)

Product Documentation

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