MicroVue YKL-40
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The MicroVue YKL-40 is a test to measure YKL-40, also known as human cartilage 39 (HC gp-39), in serum and human cell cultures. Though the role of YKL-40 is unknown at present, its pattern of expression and observed associations with various disease activities suggest it plays a role in tissue remodeling.
Specifications 
| Catalog Number: | 8020 |
| Format: | ELISA |
| Tests/kit: | 96 wells/plate |
| Sample Type: | Serum, Plasma |
The Marker
YKL-40 — also known as human cartilage glycoprotein 39 (HC gp-39) is a 40 KD glycoprotein first described in whey secretions of nonlactating cows. Subsequently, its production by chondrocytes, synovial cells, activated macrophages, neutrophils, and osteosarcoma cells (MG-63) has been reported. It has both heparin and chitin binding domains and appears to be an autoantigen in rheumatoid arthritis.
Early studies suggest YKL-40 is useful for researching:- Rheumatoid Arthritis
- Early polyarthritis
- Osteoarthritis
- Giant cell arteritis
- Liver fibrosis
- Breast cancer
- Colorectal cancer
Recently, serum YKL-40 has been investigated as a new marker for cancer. Elevated serum YKL-40 is associated in many cancers studied to date, including cancer of the colon, lung, prostate, ovary, and breast.
The Assay
| Format |
|---|
| 96-well microplate with reagents sufficient to test 40 samples in duplicate |
| Species reactivity |
| Baboon, Rhesus and Cynomolgus Macaque, and Human |
| Assay steps |
| - Add 20 µL Standards, Controls, and samples - Add 100 µL Capture Solution - Incubate 60 mintues at room temperature - Wash 4 times with 1X Wash Buffer - Add 100 µL Enzyme Conjugate - Incubate 60 minutes at room temperature - Wash 4 times with 1X Wash Buffer - Add 100 µL Working Substrate Solution - Incubate 60 minutes at room temperature - Add 100 µL Stop Solution and read Optical Density at 405 nm - Data Reduction - Linear Curve Fit |
Performance Characteristics
| Method | Sandwich |
| Analyte | YKL-40 |
| Specimen | Serum, Plasma |
| Specimen Volume | 20 µL |
| LOD* | 5.4 ng/ml |
| LLOQ* | 15.6 ng/ml |
| HLOQ* | 300 ng/ml |
| Precision CVs within-run | 5.6-6.6% |
| Precision CVs between-run | 6-7% |
| Incubation time | 3 hours |
| Cross-reactivity | YKL-40/HC gp-39 100% |
