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The Lyra Influenza A+B Assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.

A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs by the use of target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs by the use of target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.

The Lyra Influenza A+B Assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.

The Lyra Influenza A+B Assay is one in a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans.

Features & Benefits

FeatureBenefit
One-step reagent set-up

Rehydration solution is simply added to the lyophilized Master Mix

Speed

Results in less than 75 minutes after extraction

Easy training

Simplified and uniform workflow with standard pipetting volumes

Refrigerated storage

No freezer needed. 2°C to 8°C storage

Long shelf life

2 years from date of manufacture

Room temperature set up

No ice or cooling block required

Flexible 96-test format

Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls

Multi-well multiplex panels

Standardized thermocycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results

Product Specifications

Product Spec NameProduct Spec Data
Sample type

Nasal swab, Nasopharyngeal swab

Time to results

Less than 75 minutes after extraction

Reagent storage conditions

2°C to 8°C

Controls storage conditions

2°C to 8°C

Positive Percent Agreement*

A: 100%; B: 98.4%

Negative Percent Agreement*

A: 98.7%; B: 95.5%

LOD

2 to 100 TCID50/mL

Sample volume needed for extraction

180 µL

Purified nucleic acid volume needed to testing

5 µL

Shelf life

24 months

*Refer to Package Insert for additional performance claims.

 

Ordering Information

Catalog NumberDescriptionKit Size / Case Size
M100

Assay Kit

96 tests
M106

Quidel Molecular Influenza A+B Control Set

10 reactions

To better serve your molecular diagnostic needs Quidel offers two instruments