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The Lyra Direct HSV 1+2/VZV Assay is a multiplex in vitro test that detects and differentiates Herpes simplex virus type 1, Herpes simplex virus type 2, and Varicella-zoster virus nucleic acids isolated and purified from cutaneous or mucocutaneous swab specimens obtained from symptomatic patients.

A multiplex real-time PCR reaction is carried out under optimized conditions in a single tube generating amplicons for HSV-1, HSV-2, VZV, and the Process Control (PRC). Identification of HSV-1, HSV-2, VZV, and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the genomes of HSV-1, HSV-2, VZV and the PRC.

The Lyra Direct HSV 1+2/VZV Assay has one-step reagent set-up and a three-step sample prep. Test results are available in less than 70 minutes. The Lyra Direct HSV 1+2/VZV Assay is available for use on Applied Biosystems® 7500 Fast Dx, QuantStudio™ Dx Real-Time PCR Instrument, and Cepheid® SmartCycler® II.

The Lyra Direct HSV 1+2/VZV Assay is one in a growing menu of PCR tests and is used as an aid in the differential diagnosis of Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) and Varicella-zoster virus (VZV) also known as Human herpes virus 3. VZV is the causative agent for chicken pox and shingles.

Features & Benefits

FeatureBenefit
One-step reagent set-up

Rehydration solution is simply added to the lyophilized Master Mix

Speed

Results in less than 60 minutes

Easy training

Simplified and uniform workflow with standard pipetting volumes

Refrigerated storage

No freezer needed. 2°C to 8°C storage

Long shelf life

2 years from date of manufacture

Room temperature set up

No ice or cooling block required

Flexible 96-test format

Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls. Sample preparation kit includes all consumables

Product Specifications

Product Spec NameProduct Spec Data
Sample type

Cutaneous or mucocutaneous swab specimens

Time to results

Less than 60 minutes

Reagent storage conditions

2°C to 8°C

Controls storage conditions

2°C to 8°C

Sample volume needed for extraction

100 µL

Purified nucleic acid volume needed to testing

5 µL

Sensitivity*

Cutaneous: HSV-1 100.0%, HSV-2 97.1%, VZV 96.3%; Mucocutaneous: HSV-1 95.1%, HSV-2 97.9%, VZV 100.0%

Specificity*

Cutaneous: HSV-1 98.8%, HSV-2 96.7%, VZV 96.4%; Mucocutaneous: HSV-1 98.2%, HSV-2 97.1%, VZV 99.3%

Shelf life

24 months

*Data for Applied Biosystems™ 7500 Fast Dx. Refer to Package Insert for additional performance claims.

Ordering Information

Catalog NumberDescriptionKit Size / Case Size
M102

Assay Kit

96 tests
M121

Quidel Molecular HSV 1+2/VZV Control Set

10 reactions

To better serve your molecular diagnostic needs Quidel offers two instruments