Search form

The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and influenza B viral RNA in nasal and nasopharyngeal swabs from patients with signs and symptoms of respiratory infection.

The Solana Influenza A+B Assay amplifies and detects viral RNA present in transport media containing nasopharyngeal or nasal swab specimens obtained from symptomatic patients.

The assay consists of two major steps: (1) specimen preparation, and (2) amplification and detection of target sequences specific to influenza A and/or influenza B using isothermal Reverse Transcriptase — Helicase-Dependent Amplification (RT-HDA) in the presence of target-specific fluorescence probes which is performed in the Solana instrument.

Results are displayed on the touchscreen, can be saved to the instrument, printed, and are capable of being sent to the LIS and exported through one of Solana’s five USB ports. Solana Influenza A+B is also supported by the power of Virena®.

Features & Benefits

FeatureBenefit
RT-HDA technology

Rapid method of isothermal nucleic acid amplification that does not require thermal cycler

Lyophilized Master Mix reagent

Easy-to-use format, just add processed sample to rehydrate

Small, easy-to-use instrument

Eliminates the need for dedicated molecular space and costly capital equipment, allowing for testing in smaller labs

CLIA moderately complex

Requires minimal training

Refrigerated storage

No freezer needed, 2˚C to 8˚C storage

Long shelf life

Currently 12 months from date of manufacture

Room temperature set up

No ice or cooling block required

Product Specifications

Product Spec NameProduct Spec Data
Sample type
Nasal swabs and nasopharyngeal swabs in transport media* obtained from patients symptomatic of respiratory infection

*BD/Copan VTM, Remel M4, Remel M4RT, Remel M5, Remel M6, or Copen eSwab
Time to results

Approximately 45 minutes

Reagent storage conditions

Lysis and Reaction Tubes; 2˚C to 8˚C

Controls storage conditions

2°C to 8°C

Sample preparation storage conditions

Specimens should be collected, transported, stored, and processed according to CLSI M41-A. Specimens should be stored at 2°C to 8°C until tested. Specimens collected in BD UTM, Remel M4, M4RT, M5, and M6 (3 mL) are stable at 2°C to 8°C for up to 9 days

Note: Specimens collected in Copan eSwab transport media are stable at 2° C to 8°C for up to 48 hours

Clinical Sensitivity (culture)
Influenza A: 98.6% (95% CI 96.8% to 99.4%)
Influenza B: 100% (95% CI 95.7% to 100%)
Clinical Specificity (culture)
Influenza A: 95.1% (95% CI 93.7% to 96.3%)
Influenza B: 99.3% (95% CI 98.7% to 99.6%)
Clinical Sensitivity (PCR)
Influenza A: 97.2% (95% CI 95.0% to 98.4%)
Influenza B: 100.0% (95% CI 95.4% to 100%)
Clinical Specificity (PCR)
Influenza A: 96.7% (95% CI 95.4% to 97.7%)
Influenza B: 98.9% (95% CI 98.2% to 99.4%)
LOD (Influenza A)
A/Taiwan/42/06: 7.5x102 TCID50/mL
A/California/07/2009: 4.7x102 TCID50/mL
A/Texas/50/2012: 6.3x100 TCID50/mL
LOD (Influenza B)
B/Brisbane/60/08: 8.5x101 TCID50/mL
B/Massachusetts/2/2012: 3.3x101 TCID50/mL

Ordering Information

Catalog NumberDescriptionKit Size / Case Size
M300

Solana Influenza A+B Assay Kit

48 tests
M106

Quidel Molecular Influenza A+B Control Set

2.0 mL

Product Documentation