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The Lyra Adenovirus Assay is a real-time PCR in vitro diagnostic assay for the qualitative detection of human Adenovirus (HAdV) viral DNA which has been isolated from nasal and nasopharyngeal swabs obtained from individuals exhibiting signs and symptoms of acute respiratory infections. The test detects, but does not differentiate, HAdV species (A, B, C, D, E, and F) or serotypes.

During the test, a multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of adenovirus (HAdV) and the PRC occur by the use of target-specific primers and fluorescent-labeled probes which hybridize conserved regions in the genomes of adenovirus and the PRC.

The Lyra Adenovirus Assay has a convenient, one-step reagent set up performed at room temperature on the bench top. Moreover, optimized cycling conditions make results available in less than 75 minutes following extraction to aid in timely diagnosis and treatment. Lyra Adenovirus RT-PCR is available for use on the Applied Biosystems®7500 Fast Dx Real-Time PCR Instrument.

The Lyra Adenovirus Assay, when used with other Lyra assays, provides the flexibility to customize respiratory panels with other Lyra Real-Time PCR Respiratory assays in order to adapt to the shifting demands caused by variable local or seasonal disease prevalence.

Features & Benefits

FeatureBenefit
One-step reagent set-up

Rehydration solution is simply added to the lyophilized Master Mix

Speed

Results in less than 75 minutes after extraction

Easy training

Simplified and uniform workflow with standard pipetting volumes

Refrigerated storage

No freezer needed. 2°C to 8°C storage

Long shelf life

2 years from date of manufacture

Room temperature set up

No ice or cooling block required

Flexible 96-test format

Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls

Multi-well multiplex panels

Standardized thermocycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results

Product Specifications

Product Spec NameProduct Spec Data
Sample type

Nasal swab, Nasopharyngeal swab

Time to results

Less than 75 minutes after extraction

Reagent storage conditions

2°C to 8°C

Controls storage conditions

2°C to 8°C

Sensitivity*

100%

Specificity*

95.5%

Positive Percent Agreement*

100%

Negative Percent Agreement*

98.7%

LOD

A/31: 8.00 x 10-2; B/3: 8.00 x 10-2; C/1: 8.00 x 10-2; D/19: 1.61 x 101; E/4: 1.00 x 100; F/41: 3.20 x 10-2 (TCID50/mL)

Sample volume needed for extraction

180 µL

Purified nucleic acid volume needed to testing

5 µL

Shelf life

24 months

*Refer to Package Insert for additional performance claims.

Ordering Information

Catalog NumberDescriptionKit Size / Case Size
M113

Assay Kit

96 tests
M110

Quidel Molecular Adenovirus Control Set

10 reactions

To better serve your molecular diagnostic needs Quidel offers the following instruments:

QuantStudio Dx Real-Time PCR Instrument

Workflow Using the Smart Cycler

Workflow Using the 7500 Series