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Helicase Dependent Amplification Assays

Helicase Dependent Amplification (HDA) is an isothermal DNA amplification method that is similar to conventional PCR, but utilizes the action of a thermostable helicase enzyme, rather than heat to separate the nucleic acids and enable labeled primers to anneal to the DNA template and elongate under the action of the polymerase.
 
This allows DNA replication to occur at a single uniform temperature (64°C) with a simple heat block, rather than a series of varying time and temperature conditions utilized by expensive real-time thermocyclers.
 
The process supplies accurate molecular test results that you can see, achieved without the need for specialized equipment or complex software for data interpretation.
 

AmpliVue

Step 1
Biotinylated primers bind to target sequences that have been separated by the helicase.

Step 2
FITC labeled DNA probes bind to the single-stranded biotinylated amplicons.

Step 3
The reaction tube is added to the Cassette and biotinylated amplicons bind to the streptavidin coated red latex beads on the label pad and are later bound to the test line coated with anti-FITC antibodies.

Solana

Step 1
Specific primers bind to target sequences that have been separated by the helicase.

Step 2
Specific DNA probes labeled with a quencher on one end and a fluorophore on the other end bind to the single-stranded biotinylated amplicons.

Step 3
Upon annealing to the amplicons, the fluorescence probes are cleaved and the fluorescence signal increases due to physical separation of fluorophore from quencher.

 

Quidel has developed the AmpliVue and Solana assays that use Helicase Dependent Amplification to provide access to all the benefits of molecular testing for any size laboratory.

 

Herpes Family Testing

Influenza