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The Sofia Influenza A+B Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow technology to detect influenza A and influenza B viral nucleoprotein antigens in nasal and nasopharyngeal swab and nasopharygeal aspirate/wash specimens taken directly from symptomatic patients.

The Sofia Influenza A+B FIA involves the disruption of influenza A and B viral antigens. To perform the rapid influenza A and B test, the patient specimen is placed in the Reagent Tube, during which time the virus particles in the specimen are disrupted, exposing internal viral nucleoproteins. After disruption, the specimen is dispensed into the Cassette sample well. From the sample well, the specimen migrates through a test strip containing various unique chemical environments. If influenza A or B viral antigens are present, they will be trapped in a specific location.

Sofia will scan the test strip and measure the immunofluorescent signal by processing the results using method-specific algorithms and display the test results (Positive, Negative, or Invalid) on its display screen. The test results can also be automatically printed on an integrated printer if this option is selected.

An internal control line helps ensure that the rapid test has been executed properly, that the kit reagents are performing appropriately, and that adequate flow has occurred through the test strip. Another fluorescent line should form at the Control position on the test strip each time a specimen or control is tested. If no control line is detected, the test will be reported as invalid by Sofia. The Sofia Influenza A+B FIA gives you accuracy and confidence in rapid testing.

Sofia Influenza A+B FIA – the easy-to-use rapid influenza test kit – is one of a growing menu of fluorescent antibody tests available for use on Sofia. This high-quality, qualitative fluorescent test can be used as an aid in the rapid differential diagnosis of acute influenza A and influenza B viral infections.

When testing with Sofia Influenza A+B FIA, a single patient sample may also be used to test with Sofia RSV FIA.