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For In Vitro Diagnostic Use.

The MicroVue DPD EIA offers a laboratory test that measures deoxypyridinoline cross-links (Dpd), a specific marker of bone resorption which is excreted in the urine. MicroVue DPD EIA provides a quantitative measure of deoxypyridinoline (Dpd), a cross-link of bone type 1 collagen released during the resorption process. Dpd is excreted unmetabolized in urine. Elevated levels of urinary Dpd indicate elevated bone resorption in individuals. MicroVue DPD is a urinary assay that provides a quantitative measure of the excretion of deoxypyridinoline (DPD) crosslinks as an indicator of bone resorption. Elevated levels of urinary DPD indicate elevated bone resorption in individuals.

Measurement of DPD is intended for use as an aid in monitoring bone resorption changes in postmenopausal women receiving hormonal or bisphosphonate antiresorptive therapies and in individuals diagnosed with osteoporosis.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Urine

Performance Characteristics

Method: Competitive
Analyte: Deoxypyridinoline cross-links
Specimen: Urine
Specimen Volume: 50 µL
Sensitivity: 1.1 nM/L
Dynamic Range: 3-300 nM
Precision CVs within-run: 4%-8%
Precision CVs between-run: 3%-5%
Incubation Time: 3 Hours
Cross-reactivity:
    Free Dpd 100%
    Free Pyd: < 1%
    Pyd/Dpd peptides: < 2.5% 
Expected Values:
    Females: 25-44 yr. premenopausal: 3.0-7.4 nmol Dpd/mmol Cr
    Males: 25-55 yr.: 2.3-5.4 nmol Dpd/mmol Cr

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate

Species Reactivity

Rat, Mouse, Rabbit, Guinea Pig, Dog, Pig, Sheep, Cow, Horse, Squirrel, Cynomolgus Monkey, Rhesus Macaque, Human Cell Culture

Assay Steps

  • Add 50 µL of 1
  • 10 diluted Standards, Controls and samples
  • Add 100 µL cold Enzyme Conjugate
  • Incubate 2 hours ± 5 minutes at 2°C to 8°C in the dark
  • Wash 3 times with 1X Wash Buffer
  • Add 150 µL room temperature Working Substrate Solution
  • Incubate 60 ± 5 minutes at room temperature
  • Add 100 µL Stop Solution and read OD at 405 nm
  • Data Reduction: 4 Parameter
  • Correct for creatinine concentration

Ordering Information

Catalog NumberDescription
8007

96 Wells/Plate