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For In Vitro Diagnostic Use.

The MicroVue BAP EIA provides a quantitative measure of bone-specific alkaline phosphatase (BAP) activity in serum. BAP is an enzyme produced by osteoblasts, the cells responsible for bone formation. The MicroVue BAP immunoassay provides a quantitative measure of bone-specific alkaline phosphatase (BAP) activity in serum as an indicator of osteoblastic activity.

Measurement of BAP is intended for use as an aid in the:

  • management of postmenopausal osteoporosis and Paget's disease;
  • monitoring of postmenopausal women on hormonal or bisphosphonate therapy;
  • prediction of skeletal response to hormonal therapy in postmenopausal women.

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Serum

Performance Characteristics

Method: Immuno-capture
Analyte: Bone alkaline phosphatase
Specimen: Serum
Specimen Volume: 20 µL
Sensitivity: 0.7 U/L
Dynamic Range: 2-140 U/L
Precision CVs within-run: 4-6%
Precision CVs between-run: 5-8%
Incubation Time: 3.5 Hours
Cross-reactivity:
    Bone AP 100% 
    Liver AP 3-8%
    Placenta AP 0%
    Intestine AP 0.4%
Expected Values:
    Females 25-44 yr. premenopausal: 11.6-29.6 U/L
    Females ≥ 45 yr. postmenopausal: 14.2-42.7 U/L
    Males ≥ 25 yr.: 15.0 - 41.3 U/L

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate

Species Reactivity

Rabbit, Pig, Dog, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture. No recognition of Rat or Mouse

Assay Steps

  • Add 125 µL Assay Buffer
  • Add 20 µL Standards, Controls, and samples; swirl
  • Incubate 3 hours ± 10 minutes at room temperature
  • Wash 4 times with 1X Wash Buffer
  • Add 150 µL room temperature Working Substrate Solution
  • Incubate 30 ± 5 minutes at room temperature
  • Add 100 µL Stop Solution and read OD at 405 nm
  • Data Reduction: Quadratic curve

Ordering Information

Catalog NumberDescription
8012

96 Wells/Plate