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For In Vitro Diagnostic Use.

The MicroVue 25-OH Vitamin D EIA is an immunocapture assay for the determination of total 25-OH Vitamin D in human serum.

Vitamin D is a group of fat-soluble secosteroids which are produced in the skin of vertebrates after exposure to ultraviolet light from the sun, available from artificial sources, or occur naturally in a small range of foods. Total 25-OH Vitamin D is a combined measurement of two major forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol); known collectively as calciferol. When synthesized in the kidneys, calciferol circulates as a hormone; regulating, among other things, the concentration of calcium and phosphate in the bloodstream, promoting the healthy mineralization, growth and remodeling of bone.

 

 

Features & Benefits

FeatureBenefit

Product Specifications

Product Spec NameProduct Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Serum

Performance Characteristics

Method: Immuno-capture
Analyte: 25-OH Vitamin D2 & D3
Specimen: Serum
Specimen Volume: 50 µL
Limit of Detection: 1.5 ng/mL
Dynamic Range: 0-133 ng/mL
Precision CVs within-run: 2.7%-5.7%
Precision CVs between-run: 4.3%-4.7%
Incubation Time: 3 Hours

About the Assay

Assay Steps

  • Add 50 µL of Standard, Control or sample
  • Add 150 µL of the Assay Buffer
  • Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 2 hours
  • Wash 3 times with 1X Wash Buffer
  • Add 200 µL of working HRP conjugate
  • Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 30 minutes
  • Wash 3 times with 1X Wash Buffer
  • Add 100 µL of the TMB Substrate
  • With appropriate cover to avoid light exposure, place the microplate(s) on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 15 minutes
  • Add 100 µL of Stop Solution and read at 450 nm
  • Data Reduction: 4 parameter logistics, or B/B0% interpolation

Ordering Information

Catalog NumberDescription
8046

96 Wells/Plate